非小细胞肺癌组织中细胞凋亡与p53蛋白表达的定量研究

Quantitative study of apoptosis and p53 protein expression in human non-small cell lung cancer tissues

目的 :观察非小细胞肺癌 (NSCLC)组织中Caspases介导的细胞凋亡与p5 3蛋白表达情况 ,探讨两者之间的相关关系和意义。方法 :临床手术切除的NSCLC标本 4 4例 ,其中鳞癌 2 0例 ,腺癌 2 4例。采用Caspase特异的单克隆抗体M30Cy toDEATH免疫组化染色显示凋亡细胞 ,计算凋亡指数 (AI) ;免疫组化法检测p5 3蛋白表达 ,用显微图像分析系统测定阳性细胞百分率 (P/A)、平均光密度 (AOD)及阳性水平指数 (PLI)。结果 :NSCLC组织中M30阳性率为 2 5 0 % ,肺鳞癌组织的M 30阳性率明显高于肺腺癌组织 (P <0 0 5 )。低分化鳞癌组织的AI高于中、高分化鳞癌 (P <0 0 5 )。NSCLC组织中 p5 3蛋白阳性率为 5 2 2 %。p5 3蛋白的PLI与癌细胞AI之间无直线相关关系。 结论 :在NSCLC组织中存在Caspases介导的细胞凋亡机制 ,且与组织学类型及组织分化程度有关。p5 3蛋白表达与癌细胞凋亡无相关关系 ,提示突变型 p5 3蛋白失去了激活Cas pases而诱导细胞凋亡的作用 ,与肺癌的发生、发展、治疗及预后有关。

Purpose To investigate the apoptosis and expression of p53 protein and their relationship in human non small cell lung cancer (NSCLC). Methods M30 CytoDEATH, an antibody specific for the human caspases family, was used to display apoptostic cells in 44 cases of NSCLC. Apoptostic index ( AI ) was calculated. The expression of p53 protein was detected by immunohistochemical method. The percentage of positive cell P/A (%),average optical density (AOD) and positive level index ( PLI ) were assessed by micro image analyzing system. Results The expression of M30 in squamous cell carcinomas (40 0%) was higher than that in adenocarcinomas (12 5%) ( P <0 05). AI in the poorly differentiated carcinomas (4 1%) was significantly higher than those in middle (0 16%) and well differentiated carcinomas (0 4%) in squamous cell carcinomas. The average positive rate of p53 protein was 52 2% in NSCLC. There was no significant correlation between the expression of M30 and p53 protein. Conclusions Caspases mediated apoptosis mainly occurs in the tissues of squamous cell carcinomas and poorly differentiated carcinomas. Overexpression of p53 protein in NSCLC suggests that mutated p53 protein may play a role in the development of NSCLC.