小鼠亚慢性暴露于百草枯后产生帕金森病样表现

Adult C57b1 Mice Could Develop Symptoms Similar to Parkinson′s Disease after the Exposure to Paraquat

目的 :观察成年C5 7b1小鼠较长时间口服百草枯对其黑质纹状体系统的影响。方法 :将 48只成年C5 7b1小鼠随机分为 3组 ,每组 16只。MPTP组 :喂食MPTP 2 0mg·kg-1;百草枯组 :喂食百草枯 10mg·kg-1;对照组 :喂食等量生理盐水。每组小鼠每日喂食相应的毒物或生理盐水 ,共 2个月。观察小鼠的自发性行为、黑质部多巴胺能神经元以及纹状体中多巴胺、5 HT及其代谢产物含量的变化。结果 :MPTP 2 0mg·kg-1体重组及百草枯 10mg·kg-1体重组C5 7BL小鼠的自发性活动减少、纹状体中DA及其代谢产物含量降低、中脑黑质细胞数量减少。结论 :成年C5 7BL小鼠长期口服大剂量的百草枯可产生类似于帕金森病的行为 。

Aim:To observe the influence of paraquat on the nigro striatal system in the adult C57b1 mice chronically exposed to paraquat. Methods:48 adult C57b1 mice were randomly divided into 3 groups and exposed to MPTP 20 mg·kg -1 body weight ( n =16), paraquat 10 mg·kg -1 body weight ( n =16) or the same amount normal saline ( n =16) taken respectively by oral administration every day for 2 months. Spontaneous motor activities were observed during the process, at the end point of the study,these mice were decapitated and the contents of dopamine (DA),serotonin (5 HT), and metabolites in striatum were analyzed,meanwhile,the dopamine neurons at the mesencephalon were observed by the method of ABC immunohistochemistry. Results:In the paraquat (10 mg·kg -1 ) and MPTP(20 mg·kg -1 ) treated mice, a marked hypoactive behavior was observed, the striatal content of DA and metabolites were reduced without affecting 5 HT, and the number of dopaminergic neurons at the midbrain was decreased. Conclusion:C57b1 mice chronically exposed to the great amount of paraquat could yield the alterations of behavior and pathology and biochemistry similar to PD.