雌激素对癫疒间大鼠中枢神经系统Fos蛋白表达的影响及其作用部位的研究

Effect of Estrogen on Fos Protein Expression in Central Nervous System of Convulsive Rats and Its Effective Regions

目的 :了解雌激素致作用的部位。方法 :利用红藻氨酸和 6 氟二乙酯致的两种大鼠癫模型 ,应用免疫组化法检测单纯致及给予雌激素后再致大鼠海马 ,大脑皮质 ,纹状体的Fos表达。结果 :两种模型单纯致组海马、皮质、纹状体Fos表达较正常组显著增高 (P <0 0 1)。给予雌二醇 (E2 )后再致 ,红藻氨酸模型中海马 ,皮质Fos表达较单纯致组增加 (P <0 0 1,P <0 0 5 ) ;而 6 氟二乙酯模型中无变化。结论 :E2 促进癫发作的敏感性不是普遍增加全脑神经元兴奋的结果 。

Aim:To explore the effective regions of estrogen in central nervous system of convulsive rats. Methods:In two different models of epilepsy induced by kainic acid (KA) and flurothyl, we observed Fos expression of hippocampus, cerebral cortex and corpus striatum in the rats of convulsive group with or without estradiol(E 2) administration by means of immunohistochemistry.Results:In the rats of convulsive group induced by KA and flurothyl, the Fos expression in hippocampus, cerebral cortex and corpus striatum was significant higher than that in normal control group ( P <0 01). In the rats of convulsive group induced by KA after E 2 administration, the Fos expression was increased evidently in hippocampus and cerebral cortex respectively( P <0 01 and P <0 05) as compared with the convulsive group only induced by KA. Nevertheless,in the rats of convulsive group induced by flurothyl after E 2 administration, the Fos expression in hippocampus, cerebral cortex and corpus striatum was not increased ( P >0 05).Conclusion:E 2 facilitation of seizure susceptibility is not a result of a general increase in neuronal excitability throughout the brain, but rather reflects seixure promoting changes within the hippocampus.