干旱胁迫下长白落叶松的代谢组学分析

Metabonomic Analysis of Larix olgensis under Drought Stress

以长白落叶松为对象,采用代谢组测序技术分析干旱条件下长白落叶松的代谢物质的变化。利用PEG模拟干旱胁迫后在0、24、48、96 h取样,获得0 h与24 h、0 h与48 h、0 h与96 h、24 h与48 h、24 h与96 h、48 h与96 h共6组代谢产物对比数据。不同时间结果显示差异代谢物数量分别为68、67、64、74、11、15种,其中上调的差异代谢物数量分别为46、26、15、3、4、13,下调的差异代谢物数量为22、41、49、71、7、2,获得KEGG注释的差异代谢物数量分别为7、5、5、10、1、2。24 h对比于0 h出现上调代谢物质最多,分别注释到糖类代谢、甘油磷脂代谢、亚麻酸代谢等通路中,其它对比组结果中能获得KEGG注释的差异代谢物几乎都为发生下调的代谢物质,注释到的通路有脂肪酸、不饱和脂肪酸、角质、小檗碱和蜡质的生物合成,萜类、酮体、磷酸肌醇和甘油磷脂代谢等,而部分被注释到糖类代谢,亚麻酸代谢,a-亚麻酸代谢等通路的代谢物质前24 h上调,之后下调。

Metabolite sequencing was used to analyze the changes of metabolites in Larix olgensis under drought conditions. After PEG simulated drought stress,samples were taken at 0,24,48,96 h,and the data of metabolites of 0 h vs 24 h,0 h vs48 h,0 h vs 96 h,24 h vs 48 h,24 h vs 96 h,and 48 h vs 96 h were obtained. The number of differential metabolites were 68,67,64,74,11 and 15,respectively,the number of differential metabolites up-regulated were 46,26,15,3,4 and 13,respectively,the number of differential metabolites was down-regulated were 22,41,49,71,7 and 2,and the number of differential metabolites obtained by KEGG were 7,5,5,10,1 and 2. At 0 h vs 24 h,the most up-regulated metabolites appeared in the pathways of carbohydrate metabolism,glycerophospholipid metabolism,and linolenic acid metabolism,the other metabolites that were KEGG-annotated in other comparison groups were almost all metabolites that were down-regulated,the annotated pathways were biosynthesis of fatty acids,unsaturated fatty acids,keratin,berberine and waxes,metabolism of terpenoids,ketone bodies,phosphoinositides and glycerophospholipids,and some of the metabolites that are annotated to carbohydrate metabolism,linolenic acid metabolism,and a-linolenic acid metabolism are up-regulated before 24 h,and then down-regulated.