摘要
研究了在间歇式微波辅助萃取装置中提取阔叶十大功劳叶中的药用有效成分小檗碱。确定了萃取液中盐酸小檗碱的分析方法—— Al2 O3柱层析 -紫外分光光度法 ;分别选取了甲醇、乙醇、乙醇 -水 (V/ V=9∶ 1 )为溶剂 ;微波辐射时间为 2 min、4min、6min;处理比 (g原料 / m L溶剂 )为1∶ 40、1∶ 60、1∶ 80 ;利用正交实验设计考察了不同湿分原料的微波辅助萃取效率 ,得出了最优工艺条件 ;拍摄了典型萃取条件下萃取基体的透射电镜照片 ;
A study on microwave assisted extraction (MAE) for berberine in Mahonia bealei was carried out in batch equipment. The mechanism of the extraction related to structural changes in the glands was discussed. The extracts were analyzed at 347nm by ultraviolet visible spectrophotometry. The parameters such as the solvent types, the intensity of microwave energy, the ratio (g/mL) of material to solvent volume were studied. The efficiency of microwave assisted extraction of different humidity material was investigated and optimized by means of the three factor and three level factorial design. Electron micrographs showed the mechanism of the extractions was related to structural change of the cells.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第4期337-340,共4页
Journal of East China University of Science and Technology
基金
化学工程联合国家重点实验室资助项目 ( 99- 0 3)
