人β防御素-2(HBD-2)及LL-37在溃疡性结肠炎中的表达

The expression of human beta defensins-2(HBD-2)and LL-37 in the patients with ulcerative colitis

目的探讨溃疡性结肠炎(UC)患者病变结肠粘膜中抗菌肽HBD-2、LL-37 mRNA的表达。方法应用逆转录(RT)-PCR方法检测30例UC和20例正常结肠粘膜组织中HBD-2、LL-37 mRNA的表达。结果与正常结肠粘膜组织相比,UC组中HBD-2、LL-37 mRNA的表达水平明显升高(P<0.05)。结论 UC结肠粘膜组织中HBD-2、LL-37 mRNA表达水平的上调可能与溃疡性结肠炎的发病及进展有关。

Objective To investigate the mRNA expression of antimicrobial peptide HBD-2、LL-37 in the patients with ulcerative colitis(UC). Methods The colonic mucosa of 50 UC patients and 20 normal controls. Reverse transcriptase- polymerase chai action(RT-PCR) was used to semi— quantitatively analyze the mRNA expression of HBD-2、LL-37 in the tissue samples. Results The mRNA expressions of HBD-2、LL-37 were significant higher in the UC than that in the normal controls(P < 0. 05). Conclusion These findings indicate that up- regulated expression of HBD-2and LL-37 might be related to the relapses and progression of UC.