大豆叶片蛋白响应干旱胁迫的双向电泳分析

Two-dimensional Electrophoresis Pattern Analysis of Leaf Proteins from Soybean under Drought Stress

大豆是我国重要的农作物之一,蛋白质组学技术是解析植物响应逆境胁迫的重要工具之一。为进一步推进对响应干旱胁迫的大豆叶片蛋白的鉴定工作,以大豆幼苗的叶片为研究对象,用甘露醇溶液模拟干旱胁迫,处理24 h后提取叶片蛋白粉进行双向电泳分离和图谱差异分析。结果表明:采取三氯醋酸(TCA)-丙酮沉淀法提取的叶片蛋白进行双向电泳分离能够得到稳定清晰的双向电泳蛋白图谱。采取PD-Quest软件对干旱胁迫下的大豆叶片蛋白表达图谱进行差异分析,共检测和匹配425个大豆叶片蛋白点,其中差异表达的蛋白点有101个,60个表达量上调,41个表达量下调。本研究结果表明有一部分大豆叶片蛋白响应了干旱胁迫。

Soybean is one of the important crops in China. Proteomics technology is one of the important tools to analyze plantresponse to stress. To lay the foundation for further identification of homologous proteins of soybean leaf protein in response todrought stress,the leaves of soybean seedlings were used as research objects. The mannitol solution was used to simulatedrought stress. After 24 h treatment,the leaf protein powder was extracted for two-dimensional electrophoresis separation andspectral difference analysis. The results showed that the leaf protein was extracted by trichloroacetic acid-acetone precipitationmethod for two-dimensional electrophoresis analysis to obtain a stable and clear two-dimensional electrophoresis protein map.PD-Quest software was used to analyze the protein expression profiles of soybean leaves under drought stress. A total of 425 soybean leaf protein spots were detected and matched. There were 101 differentially expressed protein spots,among which 60 were up-regulated,and 41 were down-regulated. The results of this study indicated that there were some soybean leaf proteinsresponded to drought stress.