硝酸镧对黄绿木霉及其抑制大豆核盘菌能力的影响

Effect of Lanthanum Nitrate on Trichoderma Aureoviride and Its Inhibition Ability on Sclerotinia Sclerotiorum

为研究黄绿木霉菌(Trichoderma aureoviride)的拮抗性能与硝酸镧之间的相互关系,以大豆菌核病核盘菌(Sclerotinia sclerotiorum)为指示菌,采用固体培养与液体培养两种方式,检测添加不同浓度硝酸镧对黄绿木霉菌拮抗作用的影响。结果表明:硝酸镧含量为210μg·m L-1对黄绿木霉菌菌丝生长出现抑制,达到1 000μg·m L-1时可完全抑制黄绿木霉菌的生长,但低于180μg·m L-1的硝酸镧浓度可刺激黄绿木霉菌的生长;硝酸镧浓度高于330μg·m L-1对大豆菌核病核盘菌菌丝生长出现抑制,达到1 100μg·m L-1时可完全抑制大豆菌核病核盘菌的生长。一定范围内,硝酸镧的添加对黄绿木霉菌拮抗大豆菌核病核盘菌无影响,对峙培养抑菌率仍可达100%;发酵中添加硝酸镧后,粗提物抑菌能力不变,但拮抗带明显加强。

In order to study the relationship between Trichoderma aureoviride antagonistic properties with lanthanum nitrate,Sclerotinia sclerotiorum was taken as the indicator,using solid and liquid culture methods,the effects of different concentrations of lanthanum nitrate on T. aureoviride antagonistic ability were detected. The results showed that the inhibited contention of lanthanum nitrate on the mycelial growth of T. aureoviride was 210 μg·m L-1,and the completely inhibited concentration was 1 000 μg·m L-1,but the concentration below 180 μg·m L-1 could stimulate the growth of mycelium. The inhibited concentration of lanthanum nitrate on the mycelial growth of S. sclerotiorum was higher than 330 μg·m L-1,the completely inhibited concentration was 1 100 μg·m L-1. Within a certain range,the addition of lanthanum nitrate has no inhibition effect of T. aureoviride on S. sclerotiorum,the inhibition rate under confrontation culture was still up to 100%. After the addition of lanthanum nitrate in fermentation,the inhibition ability of crude extracts remained unchanged,but the antagonistic bands were obviously strengthened.