增生性瘢痕形成过程中自噬相关蛋白LC3、P62及Beclin-1的表达及临床意义

Expression and clinical significance of autophagy-related proteins LC3,P62 and Beclin-1 in hypertrophic scar formation

目的探究增生性瘢痕(HS)组织与正常皮肤组织中自噬相关蛋白表达水平的差异,并通过兔耳增生性瘢痕模型进一步探讨增生性瘢痕形成过程与自噬相关蛋白表达的关系。方法收集增生性瘢痕患者30例,各切取1处增生性瘢痕组织、1处正常皮肤组织,通过免疫组化、Western blot检测各组织标本中LC3、P62及Beclin-1的蛋白表达;采用Western blot分别检测4、8、12、24周兔耳增生性瘢痕组织与对应的兔耳正常组织中自噬相关蛋白LC3、P62及Beclin-1的表达,并进一步探讨其临床意义。结果增生性瘢痕患者的瘢痕组织LC3、Beclin-1的表达显著强于正常皮肤组织中的表达(P <0. 05),P62的表达显著弱于正常皮肤组织中的表达(P <0. 05)。兔耳增生性瘢痕模型HS形成过程中,LC3蛋白表达量逐渐升高; P62蛋白表达量逐渐下降; Beclin-1蛋白表达量均高于兔耳正常组织,差异均有统计学意义(P <0. 05)。结论人体增生性瘢痕组织中LC3、Beclin-1的表达量高于正常组织,P62的表达量低于正常组织,即增生性瘢痕形成过程与自噬相关蛋白的表达有关。

Objective To explore the differences in autophagic expression levels between hypertrophic scar(HS)tissue and normal skin tissue,and further investigate the relationship between hypertrophic scar formation and autophagy protein expression through the rabbit ear hypertrophic scar model.Methods 30 patients with hypertrophic scar were collected.One hypertrophic scar tissue and one normal skin tissue were harvested.The relative expressions of LC3,P62 and Beclin-1 in each tissue specimen were detected by immunohistochemistry and Western blot.Western blot was used to detect the autophagic-associated protein LC3(MAPLC3),P62 and Beclin-1 in the hypertrophic scar tissue of rabbit ear and the corresponding normal tissue of rabbit ears at 4 weeks,8 weeks,12 weeks,and 24 weeks,and further explore their clinical significance.Results In vivo,the expression of hypertrophic scar tissue protein LC3 and Beclin-1 was significantly stronger than that in normal skin tissue(P<0.05).The expression of P62 was significantly weaker than that in normal skin tissue(P<0.05).In animal experiments,during the process of HS formation,the protein expression of LC3 gradually increased,while the protein expression of P62 gradually decreased;the protein expression of Beclin-1 was higher than that of normal rabbit ears tissue,with statistically significant differences(P<0.05).Conclusions The expression of LC3 and Beclin-1 in human hypertrophic scar tissues is higher than that in normal tissues.While the expression of P62 is lower than that in normal tissues.That is,the expression of autophagy in human hypertrophic scar tissue showed an upward trend in a certain period of time,and was significantly higher than that in normal tissue.