长链非编码RNA AL136359.1通过上调AKR1B10的表达对胆管癌细胞增殖和迁移的影响

Effect of long-chain non-coding RNA AL136359.1 on the proliferation and migration of cholangiocarcinoma cells by up-regulating the expression of AKR1B10

目的检测AL136359. 1在胆管癌组织及细胞中的表达改变,分析其对胆管癌细胞增殖和迁移的影响。方法实时荧光定量PCR(qRT-PCR)法检测8例胆管癌组织和4种胆管癌细胞株(QBC939、RBE、HCCC-9810和TFK-1)中AL136359. 1的表达。在表达最低的胆管癌细胞株转染携带AL136359. 1的质粒增加AL136359. 1的表达,qRT-PCR验证转染效率。qRT-PCR和Western blot检测醛酮还原酶1B10(AKR1B10) mRNA和蛋白的表达。细胞计数试剂盒(CCK-8)和Transwell迁移实验检测AL136359. 1高表达对胆管癌细胞增殖能力和迁移能力的影响。结果 AL136359. 1在胆管癌组织的表达(0. 41±0. 05)明显低于癌旁组织(2. 94±0. 44,P <0. 01),在胆管癌细胞株的表达明显低于人正常胆管上皮细胞(P <0. 01),在QBC939细胞的表达最低(P <0. 01)。AL136359. 1高表达可引起AKR1B10 mRNA和蛋白表达升高(P <0. 01)。AL136359. 1高表达可明显抑制胆管癌细胞的增殖能力和迁移能力(P <0. 01)。结论 AL136359. 1在胆管癌组织和细胞株中表达减少,AL136359. 1高表达可上调AKR1B10基因的表达,参与抑制胆管癌QBC939细胞的增殖和迁移。

Objective To detect the expression of AL136359.1 in cholangiocarcinoma tissues and cells and analyze its effect on proliferation and migration of cholangiocarcinoma cells.Methods The expression of AL136359.1 in 8 cholangiocarcinoma tissues and 4 cholangiocarcinoma cell lines(QBC939,RBE,HCCC-9810 and TFK-1)was detected by real-time quantitative PCR(qRT-PCR).Transfection of plasmid carrying AL136359.1 in the cholangiocarcinoma cell line with the lowest expression level of AL136359.1 were used to increase the expression of AL136359.1,and the transfection efficiency was verified by qRT-PCR.qRT-PCR and Western blot were used to detect the expression of AKR1 B10 at mRNA and protein levels.The cell counting kit(CCK-8)and transwell migration assay were used to detect the effect of high expression of AL136359.1 on the proliferation and migration ability of cholangiocarcinoma cells.Results The expression of AL136359.1 in cholangiocarcinoma tissues(0.41±0.05)was significantly lower than that in adjacent tissues(2.94±0.44,P<0.01).The expression of AL136359.1 in cholangiocarcinoma cells was significantly lower than that in human normal cholangioepithelial cells(P<0.01),and the QBC939 cells had the lowest expression(P<0.01).High expression of AL136359.1 caused an increase in the expression of AKR1 B10 at mRNA and protein levels(P<0.01).High expression of AL136359.1 could significantly inhibit the proliferation and migration of cholangiocarcinoma cells(P<0.01).Conclusions The expression of AL136359.1 were decreased in cholangiocarcinoma tissues and cell line.The high expression of AL136359.1 could up-regulate the mRNA expression of AKR1 B10 gene,which was involved in inhibiting the proliferation and migration of QBC939 cells.