皱纹盘鲍中国群体和日本群体的自交与杂交F_1的RAPD标记

THE RAPD MARKER OF SELF-BRED AND HYBRID PROGENY BETWEEN CHINESE AND JAPANESE POPULATIONS OF HALIOTIS DISCUS HANNAI INO

利用皱纹盘鲍中国群体和日本群体野生个体的单自交和单正反杂交获得了 4个F1家系 ,其中家系AJC、BCC、CCJ、DJJ分别为日本♀×中国♂、中国♀×中国♂、中国♀×日本♂和日本♀×日本♂组合 ,采用 2 2个引物对上述四个家系及其各自亲本个体的遗传结构进行了RAPD分析。结果表明 ,四个家系的平均杂合度 :AJC为 0 .2 32 9,BCC为 0 .1 667,CCJ为 0 .1 773,DJJ为 0 .1 649;各家系两亲本间遗传距离 :AJC为 0 .2 4 62 ,BCC为 0 .1 70 1 ,CCJ为 0 .2 1 0 9,DJJ为 0 .1 688。各家系子代群体与父母本的遗传距离 :AJC为 0 .1 772和 0 .2 2 2 4 ,BCC为 0 .1 1 81和 0 .1 92 0 ,CCJ为0 .1 1 2 3和 0 .1 691 ,DJJ为 0 .0 694和 0 .1 947。各家系子代个体间的遗传距离AJC为 0 .0 976,BCC为0 .0 95 1 ,CCJ为 0 .0 699,DJJ为 0 .0 682 ,而子代各群体间遗传距离均小于 0 .0 2 2 0。

The random amplified polymorphic DNA (RAPD) technique was used to investigated the genetic differences of the abalone ( Haliotis discus hannai Ino) in 4 progeny families from different geographical populations of China and Japan: The 4 progeny families were A JC (Japanese♀×Chinese♂), B CC (Chinese♀×Chinese♂), C CJ (Chinese♀×Japanese♂), D JJ (Japanese♀×Japanese♂). The average heterozygous rates of 4 families was: A JC 0.2329, B CC 0.1667 , C CJ 0.1773, D JJ 0.1649, showed that the heterozygous rates in the hybrid progeny populations were higher than the self bred and the higher might be cause heterosis. The genetic distance between parents in 4 families were: A JC 0.2462 , B CC 0.1701, C CJ 0.2109, D JJ 0.1688, showed that the genetic distance between the two individuals from different geographic populations was longer than that from the same geographical populations, and this was the reason leading to higher heterozygous rates in their progeny, and the facts of genetic distances between wild individuals either from China or from Japan were all longer indicate that the wild resources of abalone were protected very well. The genetic distances between the progeny populations and its male/female parent were: A JC 0.1772 and 0.2224 , B CC 0.1181 and 0.1920 , C CJ 0.1123 and 0.1691, D JJ 0.0694 and 0.1947, these showed that the abalone progeny inherited more genetic material from its male parent. The genetic distances within the progeny populations were: A JC 0.0976, B CC 0.0951, C CJ 0.0699, D JJ 0.0682 and the genetic distances were all lower than 0.0220. These show that the genetic variation within and among the progeny populations were all extremely lower and indicating that the hybrid progeny was avoided to be used as the parents.