牙种植体龈沟液中IL-1β、IL-6、TNF-α的检测分析

Analysis of IL-1β、IL-6、and TNF-α in the peri-implants crevicular fluid

目的 :研究口腔种植体周围龈沟液 (PICF)中IL - 1β、IL - 6 ,TNF -α的表达特点。 方法 :34位接受种植义齿治疗的患者作为受试者 ,检测种植体数目为 4 3枚 ,其中种植体龈组织有炎症表现的 31枚作为实验组 ,龈组织健康的 12枚作为对照组。用whatman 1#滤纸条吸取种植体龈沟液 (PICF)。采用ELISA法检测PICF中的细胞因子 (IL - 1β、IL - 6 ,TNF -α)的种类及含量。 结果 :种植体龈组织健康的PICF中未检测到IL - 1β和IL - 6 ,但有少量的TNF -α。种植体龈组织有炎症表现的PICF样本中可检测到IL - 1β、IL - 6的存在 ,TNF -α的含量有显著增加。结论 :IL - 1β、IL - 6、TNF -α参与了种植体周围组织炎症的免疫调节 。

AIM:To investigate the expression of IL-1β, IL-6, and TNF-α in the peri-implants gingival crevicular fluid(PICF) from the patients with peri-implantitis. METHODS: The PICF collected from the patients with inflammatory tissues around the implants as the experimental group was examined by using of the two-site enzyme-linked immunosorbent assays(ELISA). The PICF of health implant's was collected as the contrast. RESULTS: The IL-1β,IL-6, can be detected in the PICF from inflammation of peri-implants. While they were not in the contrast group. There was TNF-α in both group, but it was significantly higher in the experimental group. CONCLUSION: The IL-1β, IL-6, and TNF-α have taken part in the immunological response in peri-implantitis, and it could be used to evaluate the healthy condition of the peri-implants.