维生素E对5/6肾切除所致慢性肾衰大鼠的保护作用

Vitamin E protected remnant kidney of 5/6 nephrectomy induced chromc renal failure(CRF)in rats

目的：观察抗氧化剂维生素Ｅ对慢性肾衰之残存肾组织和残肾功能的保护作用。方法：在５／６肾切除大鼠慢性肾衰模型上，以假手术大鼠为对照，采用形态计量和生化测定方法，对比分析术后３０、６０、９０、１２０ｄ给予或不给予维生素Ｅ治疗的慢性肾衰大鼠残肾纤维化程度，残肾功能及其活性氧化代谢状况等指标。结果：大鼠术后残存肾小球呈代偿性肥大、肾小球毛细血管代偿性增生的同时，有显著的氧自由基反应亢进、抗氧化能力减弱、肾小球硬化、滤过膜增厚、肾间质纤维化、肾组织羟脯氨酸含量增高、肾功能进行性恶化；而用维生素Ｅ治疗的大鼠，氧自由基反应和抗氧化能力趋于正常，肾小球硬化、滤过膜增厚、肾间质纤维化和肾组织羟脯氨酸含量增高的程度显著减轻，肾小球代偿性肥大和肾小球毛细血管代偿性增生的发生时间后移，肾功能恶化的速度显著减慢。结论：维生素Ｅ通过其抗氧化作用，显著抑制５／６肾切除所致慢性肾衰残肾组织纤维化，延缓残肾功能进行性恶化的速度，而对代偿性肾小球肥大和毛细血管增生无明显直接影响。

To investigate whether vitamin E could suppress the fibrosis and protect renal function of remnant kidney with chronic renal failure (CRF)in rats.Methods:120 Wistar rats were divided into 3 groups.Rats of group A were sham operated as negative control.Rats of group B had 5/6 nephrectomy as positive control.Rats of group C were operated as group B and received treatment of vitamin E in their food.The renal morphometry,hydroxyproline,renal function and oxygen free radicals were measured on 30,60,90,120 days after operation.Results:In group B,accompanied with compensatory glomerular hypertrophy and glomerular capillary proliferation,there were ①obvious glomerular sclerosis,filtration membrane thickening and interstitial fibrosis,②marked increase of renal tissue lipid peroxide and hydroxyproline content,plasma creatinine and urea concentration,urine protein concentration,and mean arterial blood pressure,and ③significant decrease of hemoglobin content, hematocrit,renal tissue vitamin E content,renal tissue superoxide dismutase and catalase activity,as compared with group A.In group C,there were decrease of renal lipid peroxide,and increase of renal vitamin E content,renal superoxide dismutase and catalase activity,presented significantly less glomerular sclerosis,filtration membrane thickening,interstitial fibrosis, and renal hydroxyproline content than group B.As a whole,all indices of renal function became better than in group B.Conclusions:Vitamin E may suppress fibrosis of remnant kidney,but does not directly affect the compensatory glomerular hypertrophy and glomerular capillary proliferation.Therefore,it may protect the function of remnant kidney in 5/6 nephrectony induced CRF rats,probably due to its antioxidant roles.