LZ-410盼生安对BEL-7402肝癌细胞作用的形态计量学研究

The effect of LZ-410 panshengan on the morphometric parameters of BEL-7402 hepatocarcinoma cell line

本文应用形态计量学的方法,以氟尿嘧啶（5-Fu）作为阳性对照药物。研究了BEL-7402肝癌细胞经LZ-410盼生安作用后细胞及细胞核的15个形态计量学参数的变化。结果表明细胞及细胞核的面积、周长、最大直径、最小直径、等效直径、等效体积、核浆比均随剂量加大而减小,说明LZ-410可使细胞及细胞核明显缩小;其中细胞及细胞核等效体积随剂量增大而减小幅度最大,细胞及细胞核面积随剂量增大而减小幅度其次。细胞核各参数的降低,尤其核浆比的降低,揭示细胞恶性程度似有所降低。各参数与药后之间有较好的剂量依赖关系,呈负相关。各参数与药物时间的关系研究表明,LZ-410盼生安对肝癌细胞作用时间的长短对形态计量学参数影响不大。

The changes of 15 morphometric parameters of BEL-7402 hepatocarcinoma cell line were studied using morphometry methed after exposure to LZ-410 panshengan (took 5-Fu as positive control). It was shown that the cellular and nuclear areas, perimeter, largest and smallest diameters, equivalent diameter, equivalent volume and the ratio of nuclus to plasma (N/P) all decreased with the increase of LZ-410 panshengan dose, indicating that the cells and nuclei shrinked markedly. Among them, the cellular and nuclear equivalent volums showed the greatest extent of decrease and the next, did the cellular and nuclear areas. The decrease of different nuclear parameters, especially the decrease of N/P seemed to suggest a lower malignancy of hepatocarcinoma cells. The morphometric parameters bore a negative correlation to LZ-410 panshengan doses and were independent of the time after exposure to LZ-410 panshengan.