甲状腺肿瘤DNA含量及胞核形态计量学参数分析与随访研究

A study of DNA content, nuclear morphometric analysis and follow up on thyroid neoplasms

应用图像分析技术和形态计量学方法，对６５例甲状腺肿瘤细胞ＤＮＡ含量和形态计量学参数进行了分析，结果表明：甲状腺肿瘤细胞ＤＮＡ含量与正常对照组比较，腺瘤组无统计学意义（Ｐ＞００５），而各甲状腺癌组均有非常显著性差异（Ｐ＜００１或Ｐ＜０００１）；甲状腺癌ＤＮＡ含量的增高与组织学类型的恶性程度基本一致；甲状腺癌５年以下存活组的ＤＮＡ含量、细胞核面积和直径均高于５年以上存活组（Ｐ＜００５或Ｐ＜０００５）；５年以下存活组的异倍体细胞数明显高于５年以上存活组（Ｐ＜０００５）。

DNA content and nuclear morphometric parameters in 65 cases with thyroid neoplasms were quantitatively measured by image analysis technology. The results showed that: After comparing between DNA content of thyriod tumor group and normal control group, there was no stastistical singificance in the group of thyriod adenoma (P>0 05), but singinificant differences occured in each group of thyroid carcinomas (P<0 05 or P<0 001) Increase of DNA content in groups of thyroid carcinomas essentially was consistent with raising of malignant degree of their histological types. DNA content, nuclear area and diameter in the group of less than 5-year survival with thyroid carcinoma, were all higher than those in the group of more than 5-year survival with the same tumor (P<0 05 or P<0 005). The number of heteroploid cell in group of less than 5-year survival was evidently higher than that of the group of more than 5-year survival (P<0 005). It suggests that the above parameters seemed to be associated with prognosis of patients.