氟尿嘧啶对BEL－7402肝癌细胞作用的形态计量学研究

The morphometric study of the effect of 5-fluridine on BEL-7402 hepatocarcinoma cell line

本文应用图像分析和形态计量学的方法研究了ＢＥＬ－７４０２肝癌细胞系经氟尿嘧啶（５－Ｆｕ）作用后细胞及细胞核的１５项形态计量学参数的变化。结果表明细胞及细胞核的面积、周长、最大直径、最小直径、等效直径、等效体积和核浆比等均随剂量增加而加大，说明５－Ｆｕ可使细胞及细胞核明显增大；其中细胞及细胞核等效体积随剂量增加而增大幅度最大，细胞及细胞核面积随剂量增加而增大幅度其次。各参数与药物剂量之间有较好的依赖关系，呈正相关。

The changes of 15 morphometric parameters of BEL-7402 hapatocarcinome cell line were studied by using morphometric method after exposure to 5-Fu. The results showed that the cell and nuclear area, perimeter, largest diameter, smallest diameter, equvalent diameter, equvalent volume and radio of nuclus to plasma N/P were increased with the increase of 5-Fu doses, and it indicated the cell and nucleus expanded apparently. In all of these parameters, the increasing extent of cell and nuclear equvalent volume were the largest, and the cell and nuclear area were the second. There was a good dose-dependent relationship between each morphometerological parameter and 5-Fu dose, expressing a positive relation. There also existed definite relationships between parameters and time after exposure to 5-Fu.