云锡矿工非小细胞肺癌形态计量和DNA定量检测研究

Cellular morphometry and DNA content measurements of non-small-cell lung cancer of miners in Yunnan Tin Mine

对云南锡矿矿工（Ｍ组）、其它地区非矿工（ＮＭ组）非小细胞肺癌（ＮＳＣＬＣ）不同病变阶段及正常对照（Ｃ组）细胞进行形态计量和ＤＮＡ定量测定比较研究，采用ＣＭＩＡＳ－００７型多功能真彩色病理图像分析仪，选择１６项几何学参数，结合ＤＮＡ含量五项指标对Ｍ、ＮＭ组进行动态观察。结果表明：１云锡矿工和其它地区非矿工ＩＮＳＣＬＣ尽管致癌因素不尽相同，但其形态计量和ＤＮＡ含量测定具有相似变化，ＤＮＡ含量变化与组织学类型也无明显关系。２ＤＮＡ含量的检测是鉴别不典型增生支气管粘膜上皮与肺癌的手段之一。３肺鳞癌癌周有活跃的Ⅱ型肺泡上皮增生，ＤＮＡ定量检测表明其核酸代谢活跃，染色体增殖能力增强，故其可能也是肺鳞癌的组织起源之一。４对有Ⅱ型肺泡上皮增生的患者临床上应严密监测，以利早期预防、早期诊断、早期治疗。５应用多功能真彩色图像分析仪将形态参数和ＤＮＡ定量测定结合起来，配以多因素统计学方法，对于非小细胞肺癌发生发展过程中各个病变阶段的诊断和鉴别诊断有较高的可重复性、可靠性和客观性。

In order to characterize the features of cytological morphometry and DNA content in miners with NSCLC in Yunnan Tin Mine and non-miners in other districts, a comparative study with image analysis was made by obsevering the specimens from miners group(M), non-miners group(NM) and control group(C). 16 morphometric parameters and 5 DNA content indexs were analysised and compared. The results showed that the regulations of NSCLC of miners in Yunnan Tin Mine CO. and non-miners in other districts had a similar morphometric and biological pattern although they suffered with different carcinogens. The DNA and the type Ⅱ hyperplasia alveolar epithelium showed more active power of proliferation. Our data suggest that alveolar epithelial type Ⅱ hyperplasia is likely to be one of the originations of lung squamous cell carcinomas. Therefore the cellular morphometry and DNA content measurements may be very helpful to pathological diagnosis for lung cancer.