环孢素A对成纤维细胞表达细胞间粘附分子1的影响

Effects of cyclosporin A on the levels of intercellular adhesion molecule-1 expressed by oral fibroblasts

目的 观察环孢素A (cyclosporinA ,CSA)对体外培养的人类口腔粘膜成纤维细胞(fibroblasts ,FB)表达细胞间粘附分子 1 (intercellularadhesionmolecule 1 ,ICAM 1 )的影响 ,了解CSA治疗口腔粘膜疾病的可能机制。方法 从健康的人类口腔粘膜中培养出FB ,向培养基中加入不同浓度的CSA ,培养 48h后用细胞酶联免疫法检测FB的ICAM 1表达水平。结果 表示ICAM 1表达水平高低的A值在健康人口腔粘膜FB中为 0 32 4± 0 0 30 ,CSA在 0 0 75～ 0 30 0ng/L的范围内以浓度 效应依赖关系下调FB的ICAM 1表达水平。结论 CSA对FB表达ICAM 1的水平具有下调作用 ,提示CSA可能是通过下调ICAM 1的表达来减缓口腔粘膜的炎症反应 。

Objective To investigate the effects of cyclosporin A(CSA) on the level of intercellular adhesion molecule 1(ICAM 1) expressed by oral fibroblasts(FB). Methods The fibroblasts were obtained from normal buccal mucosa (NM FB) and cultured in vitro. Then the levels of ICAM 1expressed by fibroblasts incubated with or without CSA in the presence of 10%fetal calf serum for 48hours at 37℃ in 5%CO 2 and air were monitored by using cell based ELISA for ICAM 1. Results We demonstrated that NM FB had ICAM 1 expression( A =0 324±0 030), and after 48 hours incubated with CSA the ICAM 1 levels expressed by oral buccal mucosa fibroblasts were dose dependently decreased. Conclusions CSA reduces the levels of ICAM 1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.