淫羊藿对口腔各矿化组织破骨细胞性骨吸收的体外实验研究

In vitro study of the effects of Epimedium on osteoclastic bone resorption in various oral mineralized tissues

目的 探讨中药淫羊藿抑制破骨细胞性骨吸收的作用。方法 体外分离、培养兔破骨细胞 ,与玻片及灭活牙片共同培养 ,加入不同浓度淫羊藿注射液。HE、原位末端标记染色玻片上的破骨细胞 ,观察其形态结构的改变 ,并观察破骨细胞在牙片上形成的吸收陷窝数目及面积的变化。结果HE染色可见用药组破骨细胞胞质浓缩 ,核固缩深染 ,部分细胞出现核分裂。原位末端标记结果显示 ,用药组破骨细胞胞质皱缩 ,胞核呈棕褐色 ,胞质淡染。提示淫羊藿可诱导破骨细胞凋亡 ,抑制骨吸收。用药组与非用药组破骨细胞凋亡率差异有显著性 ,吸收陷窝数目、面积差异也有显著性 ,随浓度增加抑制作用增强。结论 淫羊藿可诱导破骨细胞凋亡 ,抑制骨吸收 。

Objective To investigated the bone resorption caused by osteoclasts and modulating functions of a Chinese herb, Epimedium on bone resorption. Methods Osteoclasts were separated from long limb bones of new born rabbits, cultured with de activated human tooth slices and glass slices, and treated with different concentrations of Epimedium. Osteoclasts on glass slices were stained by HE staining, TUNEL staining and absorption pits on tooth slices were observed by light microscope. Results HE staining shows that the Epimedium treated cells showed shrinkage of cytosol and condensation of nuclei. Some treated cells showed breakage of nuclei. TUNEL staining shows that treated cells showed shrinkage of cytosol, dark stained nuclei and light stained cytosol. These results indicate that Epimedium can induce osteoclast to commit apoptosis. Further study shows that the number and area of absorption pits formed on treated tissues are significantly different from those on control tissues. So the effect of Epimedium on bone resorption is dose dependent. Conclusions Epimedium can induce osteoclast apoptosis and inhibit bone resorption. The effect of Epimedium on bone resorption is dose dependent.