癌胚抗原重组痘苗病毒转染树突状细胞诱导CEA特异性T细胞免疫

Induction of CEA-Specific cytotoxic T lymphocytes by dendritic cells transfected with CEA-recombinant vaccinia virus

目的 研究人癌胚抗原重组痘苗病毒 (rV CEA)转染树突状细胞 (DC)在体外诱导CEA特异性的T细胞免疫。方法 将rV CEA转染外周血单个核细胞来源的DC后用于激发自体的T细胞 ,通过对T细胞的增殖及杀伤功能的检测 ,与野生型痘苗病毒 (V 76 1)转染的DC激发的T细胞进行比较。结果 经rV CEA转染的DC激活的T细胞增殖力强 ,对CEA分泌性肿瘤细胞具特异性杀伤作用。结论 rV CEA转染的DC可以诱导CEA特异性T细胞活性。

Objective To study the in vitro immune response induced by dendritic cells(DCs) transfected with CEA\|recombinant vaccinia virus (rV\|CEA).Methods DCs collected from peripheral blood monocytes were transfected with rV\|CEA and then cocultured with autologous T cells.The proliferation of the T cells and their cytotoxic activity against CEA\|secreting tumor cells were assessed and compared with those T cells induced by DCs transfected with wide type vaccinia virus (V\|761).Results DCs transfected with rV\|CEA are effective stimuli of T lymphocyte proliferation and the T cells can lysis CEA\|specific tumor cells.Conclusion DCs transfected with rV\|CEA can elicit activition of CEA\|specific T cells .