犬暴发性肝功能衰竭模型的建立

Establishment of a canine model of fulminate hepatic failure

目的 为研究原位部分肝移植对暴发性肝功能衰竭的治疗作用 ,而建立犬肝功能衰竭模型。方法 将四氯化碳 (CCl4)与等量花生油混合溶液 ,以 0 .9ml/kg腹腔注射 ,血清谷丙转氨酶 (ALT ) ,总胆红素 (TB) ,凝血酶原时间 (PT ) ,血氨 (NH4) ,血糖 (BG )监测 ,犬死亡 ,或于存活第 7d ,1 4d活杀行病理学检查。存活第 3d ,行脑电图检查 (EEG )。结果 注射CCl4 后犬呈进行性肝功能衰竭表现 ,第 72h血ALT ,TB ,NH4 明显升高 ,PT延长 ,BG降低 ,(均P <0 .0 1 ) ;脑电图检查出现异常波型。第 7d病理检查显示 :肝细胞大片溶解坏死 ,网状支架大部分存在 ,肝细胞轻度增生。暴发性肝功能衰竭形成率为 93.0 %。动物4 8～ 96h死亡率 73.0 % ,1 4d内肝损害明显恢复。动物一般表现 ,实验室及组织学检查与临床暴发性肝衰相似。结论 该模型不需手术操作 ,适合于作实验性肝移植受体及急性肝衰治疗的研究。

Objective To establish a canine model of fulminate hepatic failure ( FHF) and study the treatment of FHF by partial orthotopic liver transplatation(POLT).Methods Carbon tetrachloride (CCL4) mixed with the same amount of peanut oil in the dosage of 0.9 ml per kilogram of body weight was injected intraperitoneally to canines. ALT,tolal bilirubine(TB), PT, NH 4,and blood suger(BG) were monitored. The pathological changes were observed when the canine died,remain alived animal were killed on 7th day and 14th day respectively. EEG was performed on 3rd day after the model eslablished. Results After CCl 4 was injected, the canine showed progressive hepatic failure, ALT, TB and NH rose persistantly, PT prolonged, and BG decreased (P< 0.01) at 72 h, The pathological changes showed that massive hepatocellular dissolution and necrosis; most fibrinous processes reticularis preserved,and small hepatocellular regenerated on 7th day. EEG showed abnormal wave. The mortality rate was 73% from 48 h to 96 h , and 93% of the animals would develop FHF. The hepatic damage of the animals recovered apparently in 14th day. The presentation , laboratory and histological findings of canine model were similar to the situation of clinical FHF. Conclusions Establishment of the FHF canine model doesn't need operation,and can be used as the receipient of experimental liver transplatation .