IL-1对多发性骨髓瘤细胞粘附分子表达的影响

Regulation by IL-1 of expression of adhesion molecules on multiple myeloma cells

为探讨人骨髓瘤细胞株ＫＭ３表面粘附分子的表达及ＩＬ－１对粘附分子表达的影响，应用直接免疫荧光标记流式细胞术分析人骨髓瘤细胞株ＫＭ３细胞表面粘附分子表达，以荧光阳性率和平均荧光强度两个指标反映粘附分子的表达量，研究ＩＬ－１对ＫＭ３细胞粘附分子表达调控。发现正常情况下，ＫＭ３细胞表面表达粘附分子ＣＤ４４、ＣＤ５４、ＣＤ４９ｂ、ＣＤ４９ｅ、ＣＤ４９ｆ，不表达ＣＤ５６和ＣＤ４９ｄ；ＩＬ－１以剂量非依赖方式促进ＫＭ３细胞表面ＣＤ４４、ＣＤ５４、ＣＤ４９ｂ、ＣＤ４９ｅ、ＣＤ４９ｆ表达。

Adhesion molecule expression of human myeloma cell line KM3 was examined by direct immunofluorescent flow cytometry analysis. KM3 cell surface mainly expressed CD 44, CD 54, CD 49 b, CD 49 e and CD 49 f (positive 20%～97%), and lacked CD 56 and CD 49 d expression (positive <5%). When exogenic IL-1 was added in the KM3 culture, expressions of CD 44, CD 40 b, CD 49 e, CD 49 f and CD 54 were increased, while CD 49 d and CD 56 did not change significantly (P>0.05). No significantly difference of KM3 cell surface adhesion molecule expression was found in various levels of IL-1. These results showed that IL-1 can promote adhesion molecule expression with dose independent pattern.