应用聚合酶链反应—单链构象多态技术检测大肠癌P^(53)基因的研究

Study of P 53 gene mutation in human colorectal carcinoma with PCR-SSCP method

为探讨Ｐ５３基因突变在大肠癌发生过程中的分子机制，应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术对３０例大肠腺癌组织中Ｐ５３基因外显子５、６、７和８分别进行检测。结果发现１６例（５３３３％）大肠癌组织出现了反映Ｐ５３基因突变的异常条带。高、中、低分化腺癌中Ｐ５３基因突变率分别为３３３３％、５０％和７０％。提示Ｐ５３基因突变与大肠癌的发生和发展有关。ＰＣＲ－ＳＳＣＰ能在ＤＮＡ片段的不同部位检测ＤＮＡ多态性和点突变，且灵敏、快速。

Using polymerase chain reaction-single strand conformation polymorphism method, we detected mutations in P 53 gene exons 5,6,7 and 8 in 30 patients with colorectal adenocaricinoma. The results showed that 16 patients (55.33%) had a variant band indicating a mutation in exons 5,6,7 and 8 of P 53 gene. Among adenocarcinomas, of the high, intesmediate and low differentiation mutation percentages of P 53 gene were 33.33%, 50% and 70%, respectively. The results suggest that P 53 gene mutation is common and associated with the differentiation degrees of colorectal adenocarcinoma. It may play an important role in the progression of adenocarcinoma. PCR-SSCP is a quick and sensitive method to detect gene mutation in mass screening for study of tumor.