云锡等生产性粉尘对大鼠肺泡巨噬细胞吞噬功能的影响

An in Vitro Study on Phagocytotic Function of Pulmonary Alveolar Macrophages of Rats Treated with Different Industrial Dusts

用巨噬细胞吞噬酵母菌的方法检测吞噬粉尘后大鼠肺泡巨噬细胞功能。发现:云锡氧化矿尘、云锡硫化矿尘、香花岭矿尘、二氧化硅尘、宣威煤烟尘等五种粉尘不同程度地抑制巨噬细胞的吞噬功能。SiO2在染尘后第二周即有改变。其它生产性粉尘均在染尘后3～6周致肺泡巨噬细胞吞噬功能下降,并在第6周仍维持较低水平。结果表明:云锡等生产性粉尘对大鼠肺泡巨噬细胞的吞噬功能有抑制作用,从而使体内非特异免疫受到影响,破坏免疫监视系统,这可能是促使肺癌发生和发展的一个有利因素之一。

An in vitro study on phagocytotic function of pulmonary alveolar macrophages of rats treated with intratracheal instillation of tin mine dusts, SiO2 and coal soot was carried out. The phagocytotic function of macrophages was determined by using the method of detecting the number of macrophages which phagocytize yeast dyed with 2% methylthionine chloride for calculating the index and rate of phagocytosis. The result showed that all kinds of dusts instilled in this experiment had effects on depressing phagototic function of macrophages as compared with the untreated rats. SiO2 showed earlier effect （after 2 weeks of instillation） than the other dusts which showed their effects after 3 weeks of instillation and continued to 6th week after instillation. The result indicates that the phagocytosis suppression induced by the dusts instilled may facilitate cancer development owing to breakdown of immune surveillance.