环指蛋白8在鼻咽癌组织中的表达及其与预后的关系

Expression of RNF8 in nasopharyngeal carcinoma and its relationship with prognosis

目的探讨环指蛋白8(RNF8)在放疗前和放疗期间鼻咽癌组织中的表达情况及其与预后的关系。方法选择2008年2月至2013年2月解放军联勤保障部队第九〇〇医院(原南京军区福州总医院)鼻咽癌初发患者68例,检测鼻咽癌患者肿瘤组织中RNF8及共济失调毛细血管扩张突变蛋白(ATM)、DNA依赖蛋白激酶催化亚单位(DNA-PKcs)在放疗前和放疗期间的表达,并分析其与预后的关系。结果接受放疗后,22例RNF8表达阳性,24例ATM表达阳性,26例DNA-PKcs表达阳性,均较放疗前明显升高(P<0.01)。68例患者有20例残留和复发,总5年生存率62.5%。放疗期间RNF8表达与ATM表达有较好的一致性,Logistic回归分析结果显示放疗期间RNF8的表达及临床分期与残留和复发密切相关(P<0.05),COX回归分析显示放疗期间RNF8表达及临床分期对生存时间有显著影响(P<0.01),放疗期间RNF8阳性者5年生存率45.5%,阴性者5年生存率为77.1%。结论 RNF8是鼻咽癌产生放疗抵抗的一个重要因素,可致放疗后的残留和复发,故可作为判断鼻咽癌预后的指标。

ObjectiveTo explore the expression of RNF8 in nasopharyngeal cancer during radiotherapy and the relationship to residue,recurrence and prognosis.MethodsExpression of RNF8,ATM and DNA-PKcs was detected in 68 patients with nasopharyngeal cancer before and during radiotherapy,and the relationship of expression to residue,recurrence and prognosis was analysed.ResultsThe expression of RNF8 was positive in 22 patients,ATM in 24 patients,DNA-PKcs in 26 patients after receiving 20 Gy′s radiation.The number of positive expression patients increased after radiation(P<0.01).There were 20 patients suffering from residue and recurrence.Total five-year survival rate was 62.5%.There was a good consistency between the expression of RNF8 and that of ATM after 20 Gy′s radiation.According to Logistic Analysis,clinical stage and the expression of RNF8 after 20 Gy′s radiation was closely related to residue and recurrence(P<0.05).Cox Analysis showed that clinical stage and the expression of RNF8 after 20 Gy′s radiation affected the survival time(P<0.01).The five-year survival rate of patients whose expression of RNF8 after 20 Gy′s radiation is positive was 45.5%,while negative patients′five-year survival rate was 77.1%.ConclusionRNF8,which can result in residue and recurrence,plays an important role in radioresistance of nasopharyngeal cancer.It can be one of the markers for prognosis.