产云芝胞外多糖发酵工艺优化及抗氧化活性研究

Fermentation and Extraction of Polysaccharides from Coriolus versicolor and Its Antioxidant Activity

试验对云芝发酵生产胞外多糖的培养基进行优化并对其抗氧化活性进行研究,通过单因素试验和中心组合设计并用Design-Expert 7.0软件对实验结果进行分析,确定培养最优条件为氮源5.870g/L多价胨,碳源53.120g/L麦芽糖,起始pH值为5,温度28℃,搅拌发酵转速150r/min,多糖得率为10.098 2g/L;对发酵多糖进行提取分离和脱蛋白处理得到EPS,然后用邻二氮菲法和DPPH法测定了EPS的抗氧化活性,结果表明,EPS对.OH自由基和DPPH.自由基均具有较高的清除率,且具有较好的量效关系,说明云芝EPS具有较强的抗氧化活性。

By using single factor experiment and central composite design,the optimal culture conditions of Coriolus versicolor for polysaccharides production were determined as polyptone 5.870 g/L,maltose53.120 g/L,pH 5,temperature 28℃ shear speed 150 r/min.Under these conditions,the EPS production reached at 10.0982 g/L.Furthermore,EPS were treated by Sevag method to remove the protein and purified by gel filtration on Sepharose CL-6B chromatography.The antioxidant activity was determined by Phenanthroline-Fe2+ method and DPPH assay.The polysaccharides showed notable inhibitory activity of OH and DPPH radical.Thus,the EPS from C.versicolor may be supplied as one natural healthy food.