Clostridium saccharobutylicum利用玉米秸秆水解液发酵生产燃料丁醇

Production of Biobutanol from Corn Stover Hydrolyzate by Clostridium saccharobutylicum

以Clostridium saccharobutylicum DSM 13864为丁醇生产菌株,采用正交优化法确定了以玉米秸秆水解液为底物的最优培养基配方:CaCO32.0 g/L,(NH4)2SO41.0 g/L,K2HPO40.5 g/L,玉米浆干粉15 g/L,MnSO4.H2O 0.01 g/L。采用该最优培养基配方,在3 L发酵罐中发酵培养40 h后,总溶剂为16.1 g/L,其中丁醇10.59 g/L,发酵强度为0.40 g/(L·h),生产率为0.33 g/g。通过对发酵过程进行变温调控证实低温有利于溶剂积累,总溶剂由17.01 g/L提高至19.98 g/L。在稀释率为0.1 h-1的变温连续发酵过程中,从80 h开始进入稳定产溶剂状态并持续至269 h,该阶段平均总溶剂为12.28 g/L(其中丁醇8.50 g/L),发酵强度为1.23 g/(L·h),是变温分批发酵(D方式)的4.92倍。

In this study,butanol production from corn stover hydrolyzate by Clostridium saccharobutylicum DSM 13864 was investigated.The medium composition was optimized by orthogonal method as follows:CaCO3 2.0 g/L,(NH4)2SO4 1.0 g/L,K2HPO4 0.5 g/L,corn steep powder 15 g/L,and MnSO4·H2O 0.01 g/L.In a 3 L bioreactor,total solvent of 16.1 g/L(butanol 10.59 g/L) was reached after 40 h of fermentation,and the productivity and yield were 0.40 g/(L·h) and 0.33 g/g,respectively.Batch fermentation process using temperature-shifting demonstrates that lower temperature is beneficial to the solvent accumulation.Compared with temperature-constant process(37 ℃),total solvent was increased from 17.01 g/L to 19.98 g/L.The temperature-shifting continuous fermentation process was operated for 269 h at dilution rate of 0.1 h-1,and the solvent production entered a stable phase at around 80 h.The average solvent during the stable solvent-production stage is 12.28 g/L(including 8.50 g/L butanol),and the average solvent productivity is 1.23 g/(L·h),representing 4.92 times of temperature-shifting batch fermentation(D).