产锰过氧化物酶细菌的筛选及其对孔雀石绿脱色的研究

Screening and Decolorization of Malachite Green of a Manganses Peroxidase-Producing Bacteria

从无锡某木材厂的腐木及腐殖土的表层土样中筛选分离得到6株产锰过氧化物酶的细菌,其中产酶最优的一株菌J09经鉴定为草木犀中华根瘤菌Sinorhizobium meliloti。在优化的条件下,该菌株发酵72 h锰过氧化物酶产量达到最高值,为607.7 U/L。利用粗酶液和发酵培养液对15 mg/L的孔雀石绿进行脱色处理3 h,脱色率分别达到78.5%和89.8%,好氧条件下的脱色率高于厌氧条件的脱色率,孔雀石绿对该菌具有一定的毒性。

Six strains with manganses peroxidase-producing capability were screened from the soil samples which were collected in a Wood factory,Wuxi,China.The strain J09 which has the highest yield was identified as Sinorhizobium meliloti.The enzyme activity reached the peak of 607.7 U/L at the third day under the optimized conditions.For the crude enzyme liquid and fermentation medium with initial concentration of 15 mg/L malachite green,the decolorization rate could reach more than 75% and 85% by 3 hours respectively.Under aerobic condition the decolorization rate was higher than that under the anaerobic condition and malachite green has some toxicity to the bacteria.