基于微流控芯片的循环肿瘤细胞的分离、检测技术

The Separation,Detection of Circulating Tumor Cells Based on Microfluidic Chip

循环肿瘤细胞(Circulating Tumor Cells,CTCs)是自肿瘤原发灶或转移灶脱落进入外周血液循环的肿瘤细胞,是肿瘤远处转移的标志。CTCs有助于癌症的早期诊断、判断疗效、个体化治疗方案制订及诊断预后。随着检测技术的不断改进,CTCs检测成为临床研究的热点,其中,微流控芯片以其高通量高效率以及低成本的特点迅速发展并被广泛研究应用。该课题对CTCs最新检测方法的研究以及未来的发展趋势进行综述,特别剖析结合微流体技术和纳米技术的微流控芯片是如何被应用于循环肿瘤细胞的分离、富集以及检测的,评估各种方法的优缺点,并探讨未来循环中流细胞检测技术的难点和方式。

Circulating tumor cells(Circulating Tumor Cells, CTCs) is the primary tumor or metastasis shed into the peripheral blood circulating tumor cells, is a sign of tumor metastasis. Early diagnosis, CTCs contribute to cancer, judging curative effect of individualized treatment plan and prognosis diagnosis. With the continuous improvement of detection, CTCs detection has become a hot spot, the clinical study, microfluidic chip for its high throughput efficiency and low cost rapid development and widely application of. The research on the latest CTCs detection method and the future development trends are reviewed, especially the analysis of microfluidic chip based microfluidics and nanotechnology is how to be applied to circulating tumor cell separation, enrichment and detection, evaluation of the advantages and disadvantages of each method, and discusses the difficulties and mode of circulating flow cell detection technology future.