Ecarin测定血浆中凝血酶原的活性

Monitering of Human Plasma Prothrombin Activity with Ecarin

目的:建立一种准确、快速和灵敏度较高的检测血浆中凝血酶原活性的方法。方法:以巨齿蛇毒中的有效成分Ecarin作为凝血酶原的激活剂,分别采用Ecarin凝固时间法(ECT)和发色底物法测定血浆中的凝血酶原的活性。结果:Earin浓度在1～8 u/ml范围内,随着血浆的稀释,ECT逐渐延长,ECT(lg)和血浆稀释度(lg)之间的相关系数均在-0.943以上,P<0.01;Ecarin浓度(lg)和ECT(lg)的相关系数均在-0.957以上,P<0.01。血浆稀释度为1:50、1:100、1:200、1:400时,Ecarin发色底物法所测血浆稀释度(lg)与A值(lg)之间的相关系数γ=-0.974,P<0.01。结论:Ecarin凝固时间法和发色底物法均具有良好的灵敏度,能较好地反映血浆中凝血酶原活性的变化。

Objective: To develop a highly accurate and sensitive test for the determination of the activity of prothrombin in human plasma. Methods: Ecarin, a protease extracted from Echis carinatus venom, was used as the activator of prothrombin Ecarin clotting times (ECT) and chromogenic substrate method were used. Results: When the concentration of Ecarin was in the range of 1-8u/ml, ECT gradually prolonged with the dilution of the plasma. The correlation(γ) between ECT(lg) and plasma dilution(lg)1: 50, 1:100,1:200, 1:400, the correlation(7) between plasma dilution(lg) and absorption was more than -0. 943, P<0.01; the correlation(γ) between the concentration of Ecarin(lg) and ECT(lg) was more than -0.957,P<0.01. When the dilution of the plasma was (A) was -0. 957, P<0. 01. Conclusion: Both the ECT and chromogenic substrate method were all sensitive to determine the activity of prothrombin in plasma.