组织因子在足月妊娠羊水中的测定及临床分析

The Detection and Clinical Analysis of Tissue Factor in the Amniotic Fluid of Term Pregnancy

目的 检测正常足月妊娠羊水中是否存在组织因子 (tissue factor,TF)这一凝血反应启动因子。 方法 用 EL ISA方法分别测定正常足月妊娠 (16例 )血浆、羊水、羊水上清液及羊水沉渣中 TF抗原水平。 结果 正常足月妊娠血浆、羊水、羊水上清液、羊水沉渣中 TF抗原水平分别为(31± 9) ng/L、(4 0 4± 186 ) ng/L、(34 8± 177) ng/L、(136 0± 6 39) ng/L,三种不同成分的羊水 TF抗原水平与血浆比较 ,差异均有显著性 (P<0 .0 1) ,尤其羊水沉渣与血浆比较 ,差异有极显著性 (P<0 .0 0 1)。羊水沉渣中 TF抗原水平与未离心羊水、羊水上清液比较 ,差异亦有显著性 (P<0 .0 1)。 结论 正常足月妊娠羊水中有大量的 TF,羊水有形成分中 TF抗原水平最高 ,羊水中的 TF可能是羊水栓塞引发

Objective To determine whether tissue factor(TF) is present in the amniotic fluid of normal term pregnancy. Methods Tissue factor antigen levels in amniotic fluid, blood plasma, centrifugal supernatant and sediment of 16 normal term pregnancies were detected with immunoenzymatic method(ELISA). Results Tissue factor antigen levels in the blood plasma, the whole amniotic fluid, the centrifugal supernatant and the sediment were [(31±9) ng/L0, [(404±186) ng/L], [(348±177) ng/L] and [(1360±639) ng/L], respectively. Tissue factor antigen concentrations in three states of the amniotic sediment(P<0.001). Conclusions (1)High concentration of tissue factor antigen is present in the amniotic fluid of normal term pregnancy. (2) The concentration of tissue factor antigen of the cell-enriched amniotic sediment is the highest in the whole amniotic fluid. (3)Tissue factor in the amniotic fluid may be the trigger of disseminated intravascular coagulation(DIC) of amniotic fluid embolism.