亚低温对缺氧缺血新生鼠脑远、近期影响

Experimental study on short-and long-term influence of hypothermia on hypoxic-ischemic brain damage in neonatal rats

为研究亚低温对缺氧缺血性脑损害 (HIBD)新生大鼠的远、近期脑保护作用 ,对新生7天的SD大鼠制作HIBD模型 ,31℃亚低温干预分别持续6小时及3小时 ,采用原位末端标记技术检测脑组织中凋亡细胞数 ,并进行行为观察。结果发现新生大鼠HIBD后有近期大量凋亡损害和饥饿诱发后远期行为如空间探索能力、生存竞争能力损害 ;亚低温干预可使近期凋亡细胞数减少 ,远期损害减轻 ,31℃亚低温持续6小时比3小时效果显著。提示31℃亚低温对缺血新生鼠脑远、近期都有保护作用 。

In order to clarify the short- and long-term influence of sub-hypothermia intervention on hypoxic-ischemic brain damage(HIBD) in animal model and its possible brain-protecting effect,204 neonatal SD rats(7 days after birth),which were recruited in this study and randomly categoried into 3 different observation and control groups,were operated to successfully make the HIBD model.Afer that,the body temperature of all these rats was effectively adjusted to 31℃ in the way of cold water bath lasting for 6h and 3h.As the short-term influence index,the apoptosis cell number in cerebral tissue was counted in several couple days after operation.On the other hand,as the long-term influence,the behavioral abilities,i.e.space research ability and living competition ability,were periodically observed in different time-segments in the near future after the hungry provocated by giving different levels of pup's storing food.Through the experimental observation,it was discovered that,in neonatal rats after experimental HIBD formation,the short-term cerebral apoptosis damage was rapidly increased and the long-term behavioral abilities were evidently reduced.Very significantly,once after receiving the sub-hypothermia intervention,the cerebral apoptosis damage was greatly decreased in several couple days and the imbalan-ced behavioral abilities were obviously ameliorate in near future in all rats with experimental HIBD so as to show a short- and long-term influence.Moveover,the effect of sub-hypothermia intervention lasting for 6h was significantly better than that lasting for 3h.Thus,it might be suggested that the effect of sub-hypothermia show excellent in both short- and long-term observation in neonatal rats with HIBD and,furthermore,the more lasting time of sub-hypothermia,the lasting time of less than 3h.