逆转录酶抑制剂联合化疗药物对卵巢癌细胞株HO-8910细胞增殖的作用

The effect of retranscriptase inhibitor (AZT) combined with drug of chemotherapy on thegrowth of human ovarian cancer cell line HO-8910.

目的:探讨逆转录酶抑制剂(AZT)与卡铂、阿霉素联合用药对卵巢癌细胞株HO-8910生长的影响。方法:用MTT分析法检测各种药物对肿瘤细胞的杀伤率,流式细胞仪分析细胞周期中DNA的含量。结果:AZT与卡铂、阿霉素联合用药对细胞抑制作用明显增强,与AZT、卡铂、阿霉素单独用药组相比差异有显著性(P<0.01)。流式细胞仪分析显示,联合用药后S期细胞明显减少,DNA合成明显降低。结论:AZT可抑制肿瘤细胞增殖,联合化疗药物后抑制作用更明显,有协同作用,可作为卵巢癌化疗的增效剂。

Objective:To investigate the effects of retransci ptase inhibitor (AZT) combined with drug of chemotherapy on the proliferation of ovarian cancer cell line HO-8910. Methods: Cancer cells were treated with each kind of drug and its killing rate was analyzed by MIT method. DNA content of cell circle was determined by flow cytometry ( FCM). Results: The inhibition effect on cancer cell line HO-8910 of AZT combined with adriamycin, carboplatin was stronger than that of single-drug group (P<0.01). The circle DNA content decreased significantly by flow cytometry (FCM) assay. Conclusions:The effect of chemotherapy can be increased with AZT and this may provide a new way for therapy of ovarian cancer.