热休克蛋白70、90在子宫内膜生长控制中的作用

Role of heat shock protein 70,90 in the growth of endometrium.

目的:探讨子宫内膜中热休克蛋白(HSP)70、90表达与雌激素受体(ER)表达的关系及在内膜生长控制中的作用。方法:用免疫组化Envision法和图象分析仪检测30例正常子宫内膜、30例增生过长子宫内膜和53例子宫内膜癌中HSP70、90和ER的表达。结果:增生期内膜中HSP90、ER的表达强于分泌期,在增生过长内膜中的表达进一步增强,而内膜癌中的表达弱于增生期和增生过长内膜;分泌期内膜中HSP70的表达强于增生期内膜,内膜癌中表达较分泌期内膜更强,内膜癌中HSP90表达随病理分级升高而减弱,非子宫内膜样癌中HSP90表达弱于内膜样癌;内膜癌中HSP70表达随病理分级升高而增强,非子宫内膜样癌中HSP70表达强于子宫内膜样癌。子宫内膜中HSP90表达与ER表达呈正相关,HSP70表达与ER表达呈负相关。结论:HSP90可能促进子宫内膜增生,HSP70与子宫内膜的增生、分泌均有关,主要与子宫内膜的分泌有关。内膜癌中HSP70、90表达对癌细胞的生长有重要作用,HSP70对癌细胞的凋亡有一定的保护作用。

Objective: To investigate the relationship between the expression of heat shock protein70,90 and ER in endometrium,and the role of heat shock protein70x90 in the growth of endometrium . Methods: 30 samples of normal endometrium, 30 samples of endometrium hyperplasia and 53 samples of endometrial carcinoma were examined for HSP70,90 and ER expression using envision immunohistochemical analysis and computerized imaging analysis system. Results: Expression of HSP90 and ER in proliferative phase was stronger than that in secretory phase of normal endometrium, and became the strongest in endometrial hyperplasia, but decreased remarkably in endometrial carcinoma. Expression of HSP70 in secretory phase was stronger than that in proliferative phase, and strongly increased in endometrial carcinoma. Expression of HSP90 was decreased with the loss of differentiation while HSP70 was increased. Expression of HSP90 in nonendometrioid was lower than that in endometrioid and HSP70 expression was stronger in endometrioid. Expression of HSP90 had direct correlation with the expression of ER in endometrium and expresion of HSP70 was inversely correlated with ER expression. Conclusions: HSP90 contribute to cell proliferation of endometrium. HSF70 may have a role in the proliferative and secretory endometrium, mainly in the secretory endometrium. HSP70,90 may have important role in the growth of endometrial carcinoma. HSP70 may have a protective role in the apoptosis of cell of endometrial carcinoma.