DN一号对糖尿病肾病的治疗作用

THE THERAPEUTIC EFFECT OF DN 1 ON DIABETIC NEPHROPATHY

①目的 观察DN一号对糖尿病肾病的治疗效果 ,并对其作用机制进行探讨。②方法 采用链脲佐菌素 (STZ)单剂 (6 5mg/kg体质量 )腹腔注射方法建立糖尿病肾病大鼠模型 ,分为糖尿病肾病非治疗组 (Ⅱ组 )、糖尿病肾病治疗组 (Ⅲ组 )和正常对照组 (Ⅰ组 )。实验初始Ⅲ组大鼠每日用DN一号 5mL灌胃 ,Ⅱ组和Ⅰ组只灌入等量饮用水 ,连续 12周。定期观察大鼠体质量、肾质量、血糖、血脂、血肌酐、血内皮素 (ET)、转化生长因子 β1(TGF β1) ,2 4h尿微量清蛋白排泄率。留取大鼠模型肾组织进行光镜、电镜观察 ,并进行免疫组织学TGF β1,血管内皮细胞生长因子 (VEGF)的半定量分析。③结果 Ⅱ组大鼠第 8,12周时 2 4h尿蛋白排泄率、血脂、血糖、血ET ,TGF β1明显高于Ⅰ组大鼠 (F =4 .89～ 6 .2 5 ,q =2 .98～ 4 .72 ,P <0 .0 5 )。Ⅲ组大鼠DN一号治疗第 8,12周时 ,上述观察指标除血糖外均较Ⅱ组大鼠低 (F =4 .2 4～ 5 .98,q =3.2 0～ 4 .6 7,P <0 .0 5 )。Ⅲ组大鼠肾组织中TGF β1,VEGF表达亦较Ⅱ组低 (H =10 .2 4 ,11.4 8,t=2 .4 6 ,2 .38,P <0 .0 5 )。④结论 DN一号能减少糖尿病肾病的尿蛋白排泄 ,对糖尿病肾病的肾损伤有保护作用。DN一号对糖尿病肾病肾损伤保护作用与DN一号降低血ET ,TGF β1,VEGF的含量 ,降低?

Objective\ To evaluate the protective effect of DN 1 and study its mechanism on diabetic nephropathy in STZ induced diabetic rats.\ Methods\ A rat model of STZ induced diabetic nephropathy was used in the study. The experimental rats were divided into two groups: Treated Group (Group 3), Untreated Group(Group 2) and Normal Control Group(Group 1).DN 1 (5 mL) was given for each rat in Group 3, same volume of drinking water for rats in Groups 1 and 2 for 12 weeks. Changes of urinary albumin, ET level in plasma, creatinine, TG concentration, TGF β 1, VEGF expression levels were measured periodically. The renal tissue was investigated microscopically and electron microscopically.\ Results\ The parameters of the experiment on week 8 and week 12 were as follows:Urinary protein excretion, blood lipid, blood sugar, blood ET and TGF β 1,VEGF of Group 2 were significantly higher than those of Group 1 ( F=4.89-6.25, q=2.98-4.72, P <0.05). All the above parameters except blood sugar in Group 3 were lower than those in Group 2 ( F=4.24-5.98, q=3.20-4.67, P <0.05). The expression of TGF β 1 and VEGF in renal tissue of Group 3 was also lower than Group 2 ( H=10.24,11.48,t=2.46,2.38, P <0.05).\ Conclusion DN 1 may decrease the excretion of urinary protein in diabetic nephropathy and protect the kidney. The protection of DN 1 for kidney is related to the decrease of the content of blood ET,TGF β 1 and VEGF as well as the expression of TGF β 1.