电化学发光免疫分析法测定血清胰岛素效果评价

MEASUREMENT OF SERUM INSULIN BY ELECTROCHEMILUMINESCENT IMMUNOASSAY

①目的 探讨电化学发光免疫分析 (ECLIA)法测定血清胰岛素的效果及临床应用价值。②方法采用ECLIA法和放射免疫分析 (RIA)法分别测定糖尿病病人的血清胰岛素含量 ,并用酶联免疫法 (ELISA)测定C 肽含量 ,并对测定结果进行分析比较。③结果 ECLIA法测定胰岛素的批内与批间变异系数分别为 1.97% ,2 .5 6 % ,平均回收率是 99.5 % ;RIA法的批内与批间变异系数分别为 10 .18% ,13.2 1% ,平均回收率是 93.4 % ;E CLIA及RIA法所测胰岛素含量与ELISA法所测C 肽含量均呈正相关 (r =0 .94 ,0 .91,P均 <0 .0 1)。④结论ECLIA法测胰岛素的各技术参数优于RIA法 ,该方法能较好评价胰岛B细胞功能 ,且操作简便 ,检测速度快 ,具有重要临床应用价值。

Objective\ To evaluate the effectiveness and clinical value of the detection of serum insulin by elctrochemiluminescent immunoassay(ECLIA).\ Methods\ Serum insulin of diabetic patients was detected by ECLIA and radioimmunoassay(RIA). C peptide was measured by ELISA simultaneously. \ Results\ The coefficient of variations(CVs) of within run and between run of ECLIA was 1.97% and 2.56% respectively,while that of RIA was 10.18% and 13.21%.The average rate of recovery of ECLIA was 99.5% and RIA was 93.4%.The concentration of serum insulin measured by both ECLIA and RIA was positively correlated with c peptide ( r=0.94 , 0.91, P <0.01).\ Conclusion\ All the technical parameters of ECLIA in measuring serum insulin gain an advantage over those of RIA. It can be used to judge B cell function of pancreas. This method is valuable for clinical application as it is easy and quick to do.