水稻抗白背飞虱基因Wbph2的初步定位

Mapping the Gene Wbph2 in ARC10239 Resistant to the Whitebacked Planthopper,Sogatella furcifera in Rice

鉴定了 ARC10 2 39/明恢 6 3F2 群体对白背飞虱的抗性 ,混合 F2 感虫稻株的 DNA,构建感虫池。应用 12 9个RFL P探针 ,结合 4种限制性内切酶 ,检测抗虫亲本 ARC10 2 39、感虫亲本明恢 6 3和感虫 DNA池的多态性。检测到 6个阳性探针 ,分布在 5个染色体上 (染色体 3、6、8、11和 12 )。应用阳性探针分析了 F2 群体的 14 2个个体 ,发现抗虫基因 Wbph2与第6染色体上的标记 RZ6 6 7、RG6 4和 RG2 6 4连锁 ,其中 ,Wbph2与 RZ6 6 7的遗传距离为 2 5 .6 c M(L OD=4 .5 0 )

The F 2 population of ARC10239/Minghui 63 was screened for the resistance to the whitebacked planthopper, Sogatella furcifera. The DNA extracted from the susceptible F 2 individuals was mixed, forming a susceptible DNA bulk. The polymorphisms in the resistant parent ARC10239, the susceptible parent Minghui 63 and the susceptible DNA bulk were tested by using 129 probes in combination with four enzymes of BamHⅠ, DraⅠ, EcoR Ⅴ and HindⅢ. Six positive probes were obtained, distributing in five rice chromosomes (Chromosome 3, 6, 8, 11 and 12). The positive probes were used to screen for the F 2 population of 142 individuals, revealing that Wbph2 was linked with the markers RZ667, RG64 and RG264 on the chromosome 6. Among the markers, the distance of RZ667 to Wbph2 was 25.6 cM (LOD=4.50).