枯草芽孢杆菌A30菌株产生的拮抗肽的分离纯化与理化性质研究

Purification and Properties of Antagonistic Peptide Produced by Bacillus subtilis A30

平皿测试表明 Bacillussubtilis A30菌株对多种植物病原真菌 ,例如水稻纹枯病菌 (Rhizoctonia solani)、稻瘟病菌 (Magnaporthegrisea)等有强烈抑制作用。在小区试验中 A30菌液对水稻纹枯病也表现出了较好的防治效果。 A30菌株培养液经硫酸铵沉淀、FPL C疏水色谱、HPLC反相色谱后 ,纯化得到一种拮抗活性物质 ,命名为 P1。 P1对热稳定 ,对蛋白酶不敏感 ;茚三酮反应呈阴性 ,用酸水解后茚三酮反应和双缩脲反应呈阳性。经 L C- ESI- MS分析初步判断 P1的分子量为14 76 .

Bacillus subtilis A30 showed strong antagonistic activities to several fungal pathogens, e.g. Rhizoctonia solani, Magnaporthe grisea, etc. in vitro. A30 culture showed good control efficiency to rice sheath blight in the field. An antagonistic substance produced by A30 was purified by ammonium sulphate precipitation, FPLC and HPLC and named P1. P1 was both thermostable and proteinases stable. Its ninhydrin reaction was negative, but after acid hydrolysis, its ninhydrin reaction and biuret reaction were positive. Its molecular weight was primarily determined as 1476.7 Da according to the LC ESI MS analysis.