水稻小球菌核菌无性世代分生孢子双曲孢菌(Nakataea sigmoidea)的发现及产孢条件研究

Observation on Nakataea sigmoidea, Vegetative Progeny of Sclerotium oryzae, and Production of Conidium

报道了黑龙江省水稻小球菌核菌无性世代的分生孢子双曲孢属 (Nakataea sigmoidea Hara) ,并对其人工培养条件进行了初步探讨。结果表明 ,在黑暗条件下 ,不同的营养条件、环境条件及培养方式病原菌均不能产生分生孢子 ;在自然光条件下 ,病原菌的菌核和菌丝在水琼脂培养基上均可产生分生孢子。生长物质和微量元素均不能促进孢子产生。C/ N对菌核萌发直接产生分生孢子有影响 ,其中 C/ N为 10 / 1时产孢率最高 ,达 5 4 .2 3%。

Nakataea sigmoidea Hara, vegetative progeny of Sclerotium oryzae was reported firstly in Heilongjiang Province of China, and its artificial cultural condition was studied. Results suggested that pathogen could not produce conidia under dark condition, natural lighting could stimulate production of conidia, many conidia were produced on water agar medium under natural lighting. Growth substance and microelement could not stimulate the production of more conidia. C/N ratio had effect on sclerotium producing conidia, percentage of producing conidia was 54.23% when the ratio of C/N was 10/1.