小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用

Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion

目的观察研究C57BL/6J小鼠肾缺血再灌注期间颈部迷走神经放电变化,并介绍其迷走神经放电采集方法与技巧。方法雄性C57BL/6J小鼠8只,8~10周龄,体质量22~26 g,沿颈正中线切开皮肤,逐层分离至颈迷走神经,用铂金电极头引导神经放电并记录其放电频率;继而进行腹部手术,通过夹闭双侧肾门缺血25 min后再恢复血流30 min,该过程持续记录其迷走神经放电频率变化。结果与基础放电频率相比,肾缺血期间迷走神经放电频率由(520±52)Hz上升至(632±59)Hz(n=8,P<0.05),再灌注后15 min内放电频率进一步升高至(672±58)Hz(n=8,P<0.05),之后逐步下降至(544±51)Hz(n=8,P<0.05)。结论肾缺血再灌注期间小鼠颈部迷走神经放电频率较基础放电频率明显上升,其原因可能涉及缺血再灌注时自身的神经保护性机制。

Objective To record the frequency variations of jugular vagus discharge in mouse model of renal ischemia-reperfusion,and introduce the method and experimental skills of the recording. Methods The right cervical vagal nerve of 8 male C57 BL/6J mice( 8 ~ 10 weeks old,weighing 22 ~ 26 g) was exposed and then bluntly separated through a midline cervical incision. The vagus nerve discharge was conducted by a pair of bipolar stainless steel electrodes and then recorded. Renal ischemia was induced by bilateral renal pedicle clamping for 25 min and followed by reperfusion for 30 min. The frequency variations of vagus nerve discharge were recorded by the electrodes during the whole process. Results Compared with the basic values,the discharge frequency were increased from 520 ± 52 Hz to 632 ± 59 Hz( n = 8,P < 0. 05) in ischemic phase,and furthermore it kept in an increasing trend during 15 minutes ' reperfusion and then reached the peak( 672 ± 58 Hz,n = 8,P < 0. 05),but then was gradually decreased to 544 ± 51 Hz after reperfusion( n = 8,P < 0. 05). Conclusion The vagus discharge is significantly increased during renal ischemia-reperfusion,which might be due to neural protection in the process.