银杏组培育苗试验初报

A Preliminary Report on the Experiment on Propagation of Ginkgo biloba in Vitro

由于银杏种子存在着后熟期,所以给银杏的胚培养工作造成了一定的困难。当用已完成后熟作用的银杏种子的胚为外植体进行培养时,5天左右长出根并展开子叶,15天左右茎伸长并长出真叶,形成完整的植株。当用未完成后熟作用的银杏种子的胚为外植体进行培养时,在所选用的培养基中,以White+2.4-D5+NH_4NO_350+LH300成苗率较高。当对组培苗的茎段进行培养时。以White+NAA0.1和White+IBA0.1的生根效果较好。

The seed of Ginkgo biloba has the after-ripening stage. This causes difficulty in embryo culture. Isolated Ginkgo biloba embryo that had completed after-ripening were cultivated in the culture medium white with 0.05% of actived carbon, about 5 days later taken cotyledons and roots, about 15 days later they grew up true leaves and formed plants. Those embryos that had not completed after-ripening were cultivated in the culture medium white + 2.4-D5+NH4NO350 + LH 300, they had a greater survival rate. The optimun concentration of NAA or IBA was 0.1 ppm for the taking roots of sections of young plant.