血清sPTA1(CD226)在判别肾移植术后急性排斥反应中的作用

EVALUATION ON THE INTERRELATIONSHIP BETWEEN SERUM sPTA1 LEVEL AND ACTUE ALLOGRAFT REJEC- TION IN RENEAL TRANSPLANTAION

用夹心ELISA法测定肾移植患者血清sPTAP1水平。观察肾移植患者围手术期及急性排斥反应时血清可溶性血小板/T细胞活化抗原1（sPTA1,CD226）水平与排斥反应的相关性。结果显示,19例肾移植患者中经病理证实的排斥反应时sPTA1显著升高,经加强免疫抑制剂治疗后,血清sPTA1下降,且sPTA1水平的变化早于临床症状表现。研究表明,sPTA1是一项较为可靠的判别和监测急性排斥反应的指标,与病理检查的结果有较好的一致性,可以作为移植物急性排斥反应的监测和预警指标。

To study the interrelationship between serum sPTA1 level and actue allograft rejection in renal transplantation, solid-phase lig-and ELISA method was used to analyze serum sPTAl level in renal transplantation. Five out of 19 patients after renal transplantation were confirmed haying actue allograft rejection by pathologic examination. The level of serum sPTA1 increased remarkably and the change in serum sP-TA1 level occurred earlier than appearance of clinical symptoms and in histopathologic manifestation. It decreased rapidly after enhancement of immune therapy. The allografts did not show any signs of acute rejection by clinic symptom and/or histopathology until the activation reached to a certain level. Therefore, the level of serum sPFA1 is a credibable guideline to recognize and monitor allograft renal transplantation. Its result is consistent with that of histopathological examination.