活血合剂中阿魏酸和芍药苷的含量测定

Determination of ferulic acid and paeoniflorin in Huoxue Mixture by HPLC

目的 :建立RP HPLC法同时测定活血合剂 (丹参、当归、赤芍、丹皮、黄芪和鸡血藻 )中阿魏酸、芍药苷的含量。方法 :采用反相高效液相法。色谱条件 :阿魏酸 :ZORBAX ODS色谱柱 ;流动相 :甲醇 0 .5 %HAC水溶液 (42∶5 8) ,检测波长为 313nm ,流速 :1.0mL·min-1。芍药苷 :ZORBAX×ODS色谱柱 ;流动相 :甲醇 水 (2 5∶75 ) ,检测波长为 2 30nm ,流速 :1.0mL·min-1。结果 :阿魏酸、芍药苷在上述色谱条件下 ,分离效果理想 ,阿魏酸在 0 .12 5～ 1.0 μg范围内 ;芍药苷在 0 .2 5～ 2 .0 μg范围内 ,线性关系良好。结论 :方法简便、准确 ,重现性好 。

Objective:To establish the determination of the contents of ferulic acid and paecniflorin in Huoxue Mixture Radix Salviae Miltiorrmizae, Radix Angelicae sinensis, Radix Paeoniae Rubra cortex Moutan, Radix Astragali and caulis Spatholobi simultaneously by HPLC.Methods:The determination was carried out with ZORBAX *ODS column. Chromatographic condition of ferulic acid: the mobile phase consisted of methanol 1%HAc (42∶58) and UV detection wavelength was at 313nm. Chromatographic condition of paecniflorin: the mobile phase consisted of methanol water (25∶75) and UV detection wavelength was 230nm.Results:There was a good linear relationship between the absorption peak area and the concentration in the rang of 0.125～1.0μg for ferulic acid and 0.25～2.0μg for paecniflorin, respectively.Conclusion:The method is simple, acurate, reproducible and can be applied for content determination of Huoxue Mixture.