‘凤丹’牡丹组织培养研究

Studies on Tissue Culture of Paeonia ostii ‘Feng Dan'

以‘凤丹’牡丹芽为材料,对其外植体消毒、褐化防治、组培苗扩繁和生根技术进行研究。结果表明:用75%酒精消毒30s,0.1%升汞消毒8min消毒效果最佳,外植体的污染率和成活率分别为32.31%和64.32%;其最适初代诱导和继代增殖培养基为MS+6-BA 1.0mg·L^(-1)+NAA0.2mg·L^(-1)和MS+6-BA 1.0mg·L^(-1)+NAA 0.2mg·L^(-1)+PIC(毒莠定,Picloram)1.0mg·L^(-1),增殖系数为4.84;生根培养基为1/2MS+CaCl2220mg·L^(-1)+IBA 3.0mg·L^(-1)+NAA0.5mg·L^(-1),生根率为74.30%;低温结合3mg·L^(-1)硝酸银培养可使褐化率降至29.15%。

Buds of Paeonia ostii ‘Feng Dan'were used as explants to study its culture techniques,such as disinfection,anti-browning,proliferation and rooting.Best disinfection result could be achieved by 75%ethanol for 30 sand 0.1% HgCl2 for 8min.Under this treatment,the contamination rate reduced to 32.31%and the survival rate reached 64.32%.The most appropriate medium for explants inducing was the medium of MS+6-BA 1.0mg·L^(-1)+NAA 0.2mg·L^(-1),and the most suitable medium for propagation was MS+6-BA 1.0mg·L^(-1)+NAA 0.2mg·L^(-1)+PIC(Picloram)1.0 mg·L^(-1).The best rooting medium was1/2MS+CaCl2(220mg·L^(-1))+IBA 3.0mg·L^(-1)+NAA 0.5mg·L^(-1),on which the rooting rate reached74.30%.Browning rate could be controlled to 29.15% under the cultivation combined chilling with 3mg·L^(-1) AgNO3.