siRNA LSINCT5联合顺铂对卵巢癌SKOV3细胞增殖和凋亡的影响

Effect of siRNA LSINCT5 combined with cisplatin on the proliferation and apoptosis of ovarian cancer SKOV3 cells

目的:探讨沉默LSINCT5联合顺铂对人卵巢癌SKOV3细胞增殖及凋亡的影响。方法:选取SKOV3细胞株,采用小干扰技术将LSINCT5基因片段转入卵巢癌SKOV3细胞中,同时联合顺铂作用于卵巢癌细胞。按不同处理因素将SKOV3细胞分为5组:对照组、NC-siRNA组、顺铂组、LSINCT5-siRNA+顺铂组和LSINCT5-siRNA组。CCK-8检测转染后SKOV3细胞对顺铂的敏感性。显微镜下观察细胞的生长状况及形态变化。Western blot和RT-PCR法分别检测与凋亡相关的Caspase 3蛋白和基因表达水平。结果:CCK-8显示,3μg/ml顺铂作用于卵巢癌细胞的抑制率较为适中,故作为后续实验的处理浓度。沉默LSINCT5能显著提高卵巢癌细胞对顺铂的敏感性。LSINCT5-siRNA+顺铂组中Caspase 3蛋白和mRNA相对表达量均明显高于其他各组(P<0.05)。结论:沉默LSINCT5提高了卵巢癌细胞对顺铂的敏感性。沉默LSINCT5联合顺铂能明显抑制卵巢癌细胞的增殖,促进卵巢癌细胞凋亡。

Objective:To evaluate the effect of the silencing LSINCT5 gene combined with cisplatin on the proliferation and apoptosis of ovarian cancer SKOV3 cells.Method:Epithelial ovarian cancer SKOV3 cells were divided into 5 groups according to different treatment factors:Control group,NC-siRNA group,Cisplatin group,LSINCT5-siRNA +Cisplatin group and LSINCT5-siRNA group.LSINCT5 gene was transferred into ovarian cancer SKOV3 cells by small interfering technology.At the same time,the transfected cells were treated with chemotherapy drugs.CCK-8 was used to detect the sensitivity of transfected SKOV3 cells to cisplatin.The growth and morphological changes of the cells were observed under the microscope.Western blot and RT-PCR methods were used to respectively detect the protein and gene expression levels of apoptosis related Caspase 3.Result:The CCK-8 showed that the appropriate drug concentration was 3μg/ml according to the inhibition rate of cisplatin on ovarian cancer cells,so it was used as the treatment concentration in the following experiments.The silencing LSINCT5 gene could significantly improve the sensitivity of ovarian cancer cells to cisplatin.The relative expression of protein and mRNA in LSINCT5-siRNA combined with group was significantly higher than in other groups(P<0.05).Conclusion:The silencing LSINCT5 improves the sensitivity of ovarian cancer cells to cisplatin.It combined with cisplatin can significantly inhibit the proliferation of ovarian cancer cells,and promote the apoptosis of ovarian cancer cells.