降钙素基因相关肽免疫阳性神经纤维对骨愈合影响的研究

The Disturbance and Significance of Calcitonin Gene－related Peptide－immunoreactive Nerves during Bone Healing

目的观察降钙素基因相关肽 (CGRP)免疫阳性神经纤维在骨愈合过程中的分布特点及规律，以期揭示 CGRP与骨形成的相互关系及可能的作用机制。方法利用大鼠胫骨上段骨缺损愈合模型，取正常对照组胫骨和不同愈合时期骨痂作HE和 CGRP免疫荧光染色，镜下观察结果并计数各不同时期阳性神经纤维的数目。结果在骨愈合过程中,CGRP免疫阳性神经纤维较多地分布于骨膜、纤维肉芽组织以及新生骨组织中，而且大多定位于骨膜和骨痂中的血管周围或附近。 CGRP免疫阳性神经纤维于修复早期即开始明显增殖，至中期达到高峰，到后期则又逐渐减少。结论CGRP免疫阳性神经纤维可以参与骨愈合过程，而且作用的方式与局部的血液循环调节有关。

Objective To observe the characterization and changes of calcitonin gene－related peptide (CGRP)－immunoreactive innervation during the repair phase of bone defect, and to investigate the relation between CGRP and bone formation and the possible mechanism underlying.Methods Bone defects were made in the upper of tibias in rats, and the calluses from different healing periods including normal tibias were obtained. Utilizing immunofluorescent technique and H.E staining, we observed the results under microscope and counted the numbers of CGRP－immunoreactive nerve fibers. Results During the period of bone healing, CGRP－immunoreactive fibers were often found in the fibrous callus, new trabeculae and periosteum, most of which were seen around the vessels or nearby.The number of CGRP－immunoreactive nerve fibers increased early in the repair phase, reached the peak in the middle of this phase, and decreased gradually at the end.Conclusion CGRP－immunoreactive nerves may take part in the process of bone healing, and the mechanism by which seems to be related to the regulation of local blood flow.