消痹灵治疗骨性关节炎的机制探讨

The effect of chinese medcine Xiao Bi Ling(XBL) on chondrocyte prliferation in v itro

目的探讨消痹灵对软骨细胞增殖的影响及治疗骨性关节炎的机理。方法取传一代的软 骨细胞接种于2块96孔培养板和培养瓶中，培养24h，分8组实验：以MTT法测细胞活性及增殖 情况(光吸收值A);用考马斯亮蓝法测细胞总蛋白。结果2％～8％的消痹灵均能促进软骨细胞 增殖及增强蛋白质的合成能力。结论消痹灵可对抗基质金属蛋白酶的降解，减轻对关节软骨 细胞的损害。

Objective In order to study the effect on chondrocyte proliferation and the mechanism of treating osteoarthritis(OA) by XBL,the experimant is carried o ut.Method The first－subcuiture chondrocytes are implanted into two 96－foramen culture plates and sixteen culture flasks.After 24 hours in cubation 8 groups ar e divided into as follows:No 1 group(control)5％serum DMEM No,2～7 groups(XBL)5 ％serum,0.5％、1％、2％、4％、8％、16％XBL－DMEM are added respectively.NO 8 gro up no cell incubation.After culture of 24 and 48 hours ,two plates are detected for viablilty and proliferation respectively by MTT method(Light absorption valu e A),cell total protein is assessed by comassie Brilliatnt G－250 colormetric as say.Result 2％～8％XBL can promote the proliferation and protein synthesis of ch ondrocyte.Conclusion XBL can protec degradation from Matrix metallo protzinase a nd decrease the impairment of chondrocyte.