RT-Nested PCR检测贝类中甲肝病毒的研究

STUDY ON DETECTION OF HAV IN SHELLFISH BY RT-Nested PCR

[目的]建立灵敏的贝类中甲肝病毒检测方法[方法]由于贝类中HAV污染水平低并存在PCR抑制剂,普通RT-PCR方法检测贝类中甲肝病毒的灵敏度低。本文建立了使用RT-NestedPCR进行贝类中甲肝病毒检测的方法。[结果]此法的检测灵敏度达0.5个TCID50/1.5g样品,与普通RT-PCR相比,灵敏度提高了100倍。

To establish a sensitive method for the detection of HAV from shellfish. The low detection sensitivity of HAV in shellfish by conventional RT-PCR is mainly because of low levels of virus contamination and PCR inhibitors in shellfish. A method for the detection of HAV in shellfish by RT-Nested PCR was developed in this paper. The detection limit is 0.5 TCID50 per 1.5 g sample, which has been improved by 100 times of sensitivity compared with conventional RT-PCR. The method for the detection of HAV from shellfish is more sensitive.