肝脂消煎剂对脂肪肝大鼠肝微粒体膜流动性及心肌黄酶、丙二醛的作用

Effect of Ganzhixiao Decoction on Membrane Fluidity, DTD and MDA of Fatty Liver Rat

目的 :探讨肝脂消煎剂治疗脂肪肝药效学机制。方法 :采用高脂饮食加白酒灌胃建立大鼠脂肪肝模型 ,以东宝肝泰作对照 ,观察肝脂消煎剂对脂肪肝大鼠肝微粒体膜流动性及心肌黄酶 (DTD)、丙二醛 (MDA)的影响。结果 :模型对照组 MDA与肝微粒体膜荧光偏振度较正常对照组明显增大 ,且两者成正相关 (r =0 .974 6 ) ,表示肝脏因脂质过氧化引起膜流动性降低 ;肝脂消煎剂能降低 MDA含量及荧光偏振度 ,拮抗膜流动性的下降 ,提高肝脏DTD活性 ,均明显优于东宝肝泰。结论 :肝脂消煎剂可抑制肝微粒体脂质过氧化 ,拮抗其膜流动性降低 ,具有良好的防治脂肪肝作用。

Objective: To investigate the pharmacodynamic mechanism of Ganzhixiao decoction in treating fatty liver.Methods: High fat diet and white wine perfusate were used to establish the fatty liver rat model. The Dongbao Gantai was chosen as the control medicine. The effect of Ganzhixiao decoction on microsome membrane fluidity, DTD and MDA of fatty liver rat was observed.Results: Compared with the normal control group, MDA and fluorescence polarization degree of hepatic microsome was significantly increased in model control group. There was positive correlation between MDA and fluorescence polarization degree of hepatic microsome (r=0.974 6). Ganzhixiao decoction was more satisfactory than Dongbao Gantai in reducing the content of MDA and fluorescence polarization degree, antagonizing reduction of membrane fluidity, increasing activity of hepatic DTD. Conclusion: TCM could inhibit lipid peroxidtion of hepatic microsome, antagonize reduction of membrane fluidity, and had a satisfactory effect on preventing and treating fatty hepatitis.