健脾温中方治疗脾胃虚寒型肠易激综合征实验研究

Experimental Study on Jianpi Wenzhong Formula Treating Irritable Bowel Syndrome of Spleen-gastric Deficiency Type

目的 :探讨健脾温中方对脾胃虚寒型肠易激综合征 (IBS)的作用机制。方法 :将 5 0只 Wistar大鼠 (180±2 0 ) g,随机分为 5组 :正常组、模型组、中药大剂量组、中药小剂量组、得舒特组。分别给予健脾温中方 ,得舒特 ,0 .9% Na Cl溶液灌胃。观察体重、稀便级、碳末推进率、血浆和组织中的生长抑素 (SS)、血管活性肠肽 (VIP)的含量变化。结果 :健脾温中方明显改善脾胃虚寒型 IBS的大鼠症状体征 ,且成一定的量效关系 ,降低 SS、VIP的含量。结论 :健脾温中方可通过调整紊乱的胃肠激素分泌、释放来达到治疗目的。

Objective:To study the mechanism of Jianpi Wenzhong formula (JWF) treating irritable bowel syndrome (IBS) of spleen gastric deficiency type. Methods: Fifty wistar rats were divided randomly into 5 groups: normal group (NG), model group (MG), high dosage herb treated group (HG), low dosage herb treated group (LG) and Dicetel treated group (DG). Each group received 0.9 % NaCl, 0.9 % NaCl, Jianpi Wenzhong decoction of high dosage, Jianpi Wenzhong decoction of low dosage and Dicetel respectively. Changes in body weight, degree of diarrhea, charcoal suspension pushing rate and concentrations of somatostatin (SS) and vasoactive intestinal peptide (VIP) in plasma and intestinal tissue were determined.Results:JWF could effectively increase body weight, improve diarrhea of IBS rats of spleen gastric deficiency type and lower the increased concentrations of SS and VIP in a dose dependent manner. Conclusion: The mechanism of JWF in the treatment of IBS was to adjust the disordered secretion and release of gastrointestinal hormones.